Advanced Microscopy Facility

The multi-user Advanced Microscopy Facility at the Bosch Institute provides access to, and expert training on, basic transmitted light and fluorescence microscopes, advanced confocal microscopes and the PALM Combi System that combines laser microdissection pressure catapulting and optical tweezers in one instrument. To register to the Bosch AMF, please complete the registration form. Registered users also have access to image analysis and processing software such as MetaMorph and Bitplane Imaris Suite.

Microscopy Officer

Dr Louise Cole
Ph: 9351 2520
Email: louise.cole@bosch.org.au

Where to find the Bosch AMF

The majority of instruments in the Bosch AMF are located in the Anderson Stuart Building, F13 on the Camperdown Campus, The University of Sydney. See the map for directions.

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Featured image

Skeletal muscle fibre from an mdx (dystrophic) mouse
"Recently, we have shown that reactive oxygen species (ROS) contribute to muscle pathophysiology in mdx mice (Whitehead et al., 2008 J. Physiol 586:2003-2014). We are now investigating the role of NADPH oxidase, a membrane-bound protein complex, which produces ROS. This image shows one of the NADPH oxidase proteins, p22phox (green), which co-localises with Caveolin-3 (red) along the muscle fibre surface membrane of an mdx mouse. Nuclei are identified by DAPI staining (blue). The image was taken with the Zeiss 510 Meta Laser Scanning Confocal microscope, using a x100 oil objective.” Dr Nick Whitehead (Discipline of Physiology, Bosch Institute).