Zeiss LSM 510 Meta Spectral Confocal Microscope
In February 2007, the Bosch Institute purchased a brand new state-of-the-art laser scanning confocal microscope from Carl Zeiss Australia – the LSM 510 Meta. Funding for this microscope was supported by an NHMRC Equipment grant 2006, a University of Sydney Large Equipment grant 2007, RIBG funding and also the Rebecca L. Cooper Medical Research Foundation.
This Zeiss LSM 510 Meta is based on one of the new inverted research Zeiss AxioObserver microscopes that has a touch screen display and thereby opens a new dimension in automated operation. It is provided with a number of lasers for excitation of fluorescent dyes and fluorescent proteins. These include a 405 nm laser for UV excitation, an Argon (458, 477, 488 and 514 nm lines), 561 nm DPSS and 633 nm HeNe laser. The LSM software allows for multi-channel imaging, z-sectioning, time-lapse imaging, live-cell imaging, quantitative co-localisation, FRET analyses and photo-bleaching methods.
The unique scanning module has three conventional and one Meta detector with 32-spectral channels. The Meta detector enables Emission Fingerprinting (a technique developed by Carl Zeiss) to be carried out so that the emission spectra of different dyes is separated precisely. This can eliminate cross-talk, auto-fluorescence bleed-through and can even reliably separate dyes with close emission signals.
Manuals Available:
