Leica Light Microscopy
Location
Room S448, Anderson Stuart Building
Leica DMRBE Upright Microscope
The Leica DMRBE upright light microscope that was part of the old Leica TSCNT confocal microscope has been given a new lease of life and a new name, the Leica Stereology Microscope with the addition of a motorised xyz-stage and Stereo Investigator software for Design based Stereology, Image Analysis, and 2D Anatomical Mapping (purchased from SciTech). The Virtual Slice module also allows you to simply create and analyse high-resolution image montages. The microscope is set-up for transmitted light and basic fluorescence microscopy which includes standard DAPI, FITC and Rhodamine filters sets. Funding for the purchase of this microscope was provided by NHMRC Equipment Grant 2008 and Rebecca L. Cooper Medical Foundation Grant 2009.



Images from top to bottom: Whole virtual slice, Virtual slice software, Needle track
llumination sources
- 12V/ 100W HLX Xenophot 64625 Halogen lamp
- HBO 50W Hg short arc lamp
Reflected light filters
- UV (A 51382) - BP 340-380, DM 400, SF LP 425
- Blue (I3 513828) - BP 450-490, DM 510, SF LP 515
- Green (N2.1 513832) - BP 515-560, DM 580, SF LP 590
- 1 blank filter space
Objectives
- Plan fluotar 5x dry/ NA 0.12/ WD 0.12mm
- Plan fluotar 16x imm ph2/ NA 0.50/ WD 0.15mm
- Plan fluotar 40x oil/ NA 1.00-0.50/ WD 0.08mm
- Plan apo 63x oil/ NA 1.32-0.6/ WD 0.07mm
- Plan apo 100x oil/ NA 1.40-0.7/ WD 0.09mm
- Plan apo 100x oil ph3/ NA 1.40/ WD 0.09mm
- Plan apo 63x water corr/ NA 1.20/ WD 0.22mm
Leica DMIRBE Inverted Microscope
llumination sources
- 12V/ 100W HLX Xenophot 64625 Halogen lamp
- HBO 50W Hg short arc lamp
Reflected light filters
- UV (A 51382) - BP 340-380, DM 400, SF LP 425
- Blue (I3 513828) - BP 450-490, DM 510, SF LP 515
- Green (N2.1 513832) - BP 515-560, DM 580, SF LP 590
- 1 blank filter space
Objectives
- Neo plan 5x ph0/ NA 0.12/ WD 14.0mm
- Plan fluotar 16x imm/ NA 0.50/ WD 0.15mm
- Plan fluotar 40x oil ph3/ NA 1.00/ WD 0.08mm
- Plan apo 63x oil ph3/ NA 1.32/ WD 0.07mm
File saving
- Network
- CD
- Floppy 3.5" 1.44MB
- MO disc




