Zeiss "Deconvolution" Microscope
Location
Room S443, Anderson Stuart Building
The facility
A Zeiss "Deconvolution" Microscope System was acquired in 2001, with grants raised by the IBR. Value $110,000. The system comprises:
- Zeiss Axioplan 2 upright microscope, automated
- Zeiss AxioVision vers 4.0 image acquisition software (includes Deconvolution and 4D rendering modules)
- Zeiss AxioVision Viewer image storage software
- Zeiss AxioCam HR digital monochrome CCD camera
Zeiss Axioplan 2 Upright microscope
Illumination sources
- 12V/ 100W HLX Xenophot 64625 Halogen lamp
- HBO 103W Hg short arc lamp
NEW reflected light filters
- DAPI - 365, 395LP, 420LP
- Alexa 488 - 480/40, 505LP, 535/50
- GFP - 470/40, 495LP, 515/30
- Texas Red - 560/55, 595LP, 645/75
- CY3 - 545/30, 570LP, 610/75
- CY5 - 620/60, 660LP, 700/75
- Dual - FITC/CY3
- Triple - FITC/CY3/CY5
Objectives
- Plan Apochromat 5x dry/ NA 0.16
- Plan Neofluar 10x dry/ NA 0.3/ WD 5.6mm
- Plan Neofluar 20x dry/ NA 0.50/ WD 2.0mm
- Plan Neofluar 40x oil/ NA1.3/ WD 0.17mm
- Plan Apochromat 63x oil/NA 1.4/ WD 0.09mm
Zeiss AxioVision software
Acquisition
- Multiple-channel images
- Image z-stacks
Processing
- Processing of 14-bit image (brightness, contrast, gamma)
- Image deconvolution
- 3D-rendering (x, y, z) and 4D-rendering (x, y, z, t) with Inside 4D module
File saving
- Network
- CD or DVD+R/ RW
- Floppy 3.5" 1.44MB
- USB devices
