Zeiss "Deconvolution" microscope
Advanced Imaging Facility, Room S443, Anderson Stuart Building
Phone: +61 2 9351 3930
A Zeiss "Deconvolution" Microscope System was acquired in 2001, with grants raised by the IBR. Value $110,000. The system comprises:
- Zeiss Axioplan 2 upright microscope, automated
- Zeiss AxioVision vers 4.0 image acquisition software (includes Deconvolution and 4D rendering modules)
- Zeiss AxioVision Viewer image storage software
- Zeiss AxioCam HR digital monochrome CCD camera
Illumination sources
12V/ 100W HLX Xenophot 64625 Halogen lamp
HBO 103W Hg short arc lamp
NEW reflected light filters
DAPI - 365, 395LP, 420LP
Alexa 488 - 480/40, 505LP, 535/50
GFP - 470/40, 495LP, 515/30
Texas Red - 560/55, 595LP, 645/75
CY3 - 545/30, 570LP, 610/75
CY5 - 620/60, 660LP, 700/75
Dual - FITC/CY3
Triple - FITC/CY3/CY5
Objectives
Plan Apochromat 5x dry/ NA 0.16
Plan Neofluar 10x dry/ NA 0.3/ WD 5.6mm
Plan Neofluar 20x dry/ NA 0.50/ WD 2.0mm
Plan Neofluar 40x oil/ NA1.3/ WD 0.17mm
Plan Apochromat 63x oil/NA 1.4/ WD 0.09mm
Acquisition
Multiple-channel images
Image z-stacks
Processing
Processing of 14-bit image (brightness, contrast, gamma)
Image deconvolution
3D-rendering (x, y, z) and 4D-rendering (x, y, z, t) with Inside 4D module
Network
CD or DVD+R/ RW
Floppy 3.5" 1.44MB
USB devices
