Bosch Institute
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Live Cell Imaging Facility

The facililty

 

The system comprises:

This system is currently being re-located to Room N135, Anderson Stuart Building.

Fluorescence system

 

The Sutter DG-4 Wavelength Switcher is a combined Xenon Arc lamp and high speed wavelength switcher, ideal for fast ion imaging where image ratios at different excitation wavelengths are required at high frame rates. It comprises:

  • Built-in 175W ozone-free Xenon lamp
  • Uses up to 4 standard (25mm diameter) interference filters
  • Beam is switched between filters in less than 1.2 ms
  • Allows video-rate ratio imaging (depending on camera hardware)
  • Liquid light guide provides vibration isolation from microscope
  • Integral shuttering and neutral density filtering

Filters
The system is currently configured for ratio imaging of:

  • FURA-2 (Omega filter XF04: Exc1 340AF15, Exc2 380AF15, DM 415 DCLP, Em 510WB40).
  • Di-8-ANEPPS (Omega filter XF17: Exc1 490DF20, Exc2 440AF21, DM 550DCLP, Em 660DF50)

Software

  • Fluorescence image acquisition is controlled by MetaFluor (Universal Imaging)
  • Image processing is achieved with MetaMorph (Universal Imaging)

File saving

 
  • Floppy 3.5" 1.44MB (confocal data)
  • DVD and CD burning (fluorescence data)
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Dr Louise Cole
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