Live Cell Imaging Facility

The system comprises:

• Olympus IX70 inverted microscope
• Fluorescence System
• MetaFluor and MetaMorph software for fluorescence image acquisition.
• Hamamatsu multi-format cooled CCD camera
• TMC Isolation table

This system is currently being re-located to Room N135, Anderson Stuart Building.

The Sutter DG-4 Wavelength Switcher is a combined Xenon Arc lamp and high speed wavelength switcher, ideal for fast ion imaging where image ratios at different excitation wavelengths are required at high frame rates. It comprises:

• Built-in 175W ozone-free Xenon lamp
• Uses up to 4 standard (25mm diameter) interference filters
• Beam is switched between filters in less than 1.2 ms
• Allows video-rate ratio imaging (depending on camera hardware)
• Liquid light guide provides vibration isolation from microscope
• Integral shuttering and neutral density filtering

Filters
The system is currently configured for ratio imaging of:

• FURA-2 (Omega filter XF04: Exc1 340AF15, Exc2 380AF15, DM 415 DCLP, Em 510WB40).
• Di-8-ANEPPS (Omega filter XF17: Exc1 490DF20, Exc2 440AF21, DM 550DCLP, Em 660DF50)

Software

• Fluorescence image acquisition is controlled by MetaFluor (Universal Imaging)
• Image processing is achieved with MetaMorph (Universal Imaging)

• Floppy 3.5" 1.44MB (confocal data)
• DVD and CD burning (fluorescence data)