Professor David I Cook

E:  david.cook@bosch.org.au T:  +61 2 9036 3314 F:  +61 2 9036 3139 K25 - Medical Foundation Building The University of Sydney NSW 2006 Australia

• Research interests
• Competitive grants
• Publications

Research interests

Current national competitive grants

Publications

2007

Lee, I, Dinudom, A, Sanchez-Perez, A, Kumar, S, Cook, D. Akt mediates the effect of insulin on epithelial sodium channels by inhibiting Nedd4-2. The Journal of biological chemistry. 2007; 282:29866-73

Sanchez-Perez, A, Kumar, S, Cook, D. GRK2 interacts with and phosphorylates Nedd4 and Nedd4-2. Biochemical and biophysical research communications. 2007; 359:611-615

Kunzelmann, K, Sun, J, Meanger, J, King, N, Cook, D. Inhibition of airway Na+ transport by respiratory syncytial virus. Journal of virology. 2007; 81:3714-20

Dinudom, A, Rauh, R, Korbmacher, C, Cook, D, Farman, N, Lee, I, Wielputz, M, Boulkroun, S. (NDRG2) stimulates amiloride-sensitive Na+ currents in Xenopus laevis oocytes and fisher rat thyroid cells. JOURNAL OF BIOLOGICAL CHEMISTRY. 2007; 282:28264-28273

Cook, D, Gebski, V, Keech, A. Subgroup analysis in clinical trials. In: Interpreting and reporting clinical trials: A guide to the CONSORT statement and the principles of randomised controlled trials. Australia: Australasian Medical Publishing Company Ltd. 2007. p. 105-113.

Keech, A, Wonders, S, Cook, D, Pike, R, Gebski, V. Balancing the outcomes: reporting harms (adverse events). In: Interpreting and reporting clinical trials: A guide to the CONSORT statement and the principles of randomised controlled trials. Australia: Australasian Medical Publishing Company Ltd. 2007. p. 132-142.

2006

Arthur, J, Sanchez-Perez, A, Cook, D. Scoring of predicted GRK2 phosphorylation sites in Nedd4-2. Bioinformatics (Oxford, England). 2006; 22:2192-5

Rauh, R, Dinudom, A, Fotia, A, Paulides, M, Kumar, S, Korbmacher, C, Cook, D. Stimulation of the epithelial sodium channel (ENaC) by the serum- and glucocorticoid-inducible kinase (Sgk) involves the PY motifs of the channel but is independent of sodium feedback inhibition. Pflugers Archiv-European Journal of Physiology. 2006; 452:290-299

Fotia, A, Cook, D, Kumar, S. The ubiquitin-protein ligases Nedd4 and Nedd4-2 show similar ubiquitin-conjugating enzyme specificities. International Journal of Biochemistry & Cell Biology. 2006; 38:472-479

2005

Hu, H, Cook, D. Phospholipase C beta isoforms mediating the CCh-induced calcium response. Faseb Journal. 2005; 19:A1664-A1664

Hryciw, D, Ekberg, J, Lee, A, Lensink, , Kumar, S, Guggino, W, Cook, D, Pollock, C, Poronnik, P. Nedd4-2 is required for constitutive albumin endocytosis in proximal tubule cells. Faseb Journal. 2005; 19:A1182-A1182

Ekberg, J, Fotia, A, Adams, D, Cook, D, Kumar, S, Poronnik, P. Sglk-1 and Nedd4/4-2 regulation of voltage-sensitive sodium channels. Faseb Journal. 2005; 19:A1158-A1158

Day, M, Bailey, C, Rasko, J, Johnson, M, Cook, D. Maintenance of potassium channel activity prevents cell division in the mouse preimplantation embryo. Biology of Reproduction. 2005; SI:110-110

2004

Hryciw, D, Ekberg, J, Lee, A, Lensink, I, Kumar, S, Guggino, W, Cook, D, Pollock, C, Poronnik, P. Nedd4-2 functionally interacts with ClC-5: involvement in constitutive albumin endocytosis in proximal tubule cells. The Journal of biological chemistry. 2004; 279:54996-5007

Kunzelmann, K, König, J, Sun, J, Markovich, D, King, N, Karupiah, G, Young, J, Cook, D. Acute effects of parainfluenza virus on epithelial electrolyte transport. The Journal of biological chemistry. 2004; 279:48760-6

Keech, A, Wonders, S, Cook, D, Gebski, V. Balancing the outcomes: reporting adverse events. The Medical journal of Australia. 2004; 181:215-8

Dinudom, A, Fotia, A, Lefkowitz, R, Young, J, Kumar, S, Cook, D. The kinase Grk2 regulates Nedd4/Nedd4-2-dependent control of epithelial Na+ channels. Proceedings of the National Academy of Sciences of the United States of America. 2004; 101:11886-90

Fotia, A, Ekberg, J, Adams, D, Cook, D, Poronnik, P, Kumar, S. Regulation of neuronal voltage-gated sodium channels by the ubiquitin-protein ligases Nedd4 and Nedd4-2. The Journal of biological chemistry. 2004; 279:28930-5

Winston, N, Johnson, M, McConnell, J, Cook, D, Day, M. Expression and role of the ether-a-go-go-related (MERG1A) potassium-channel protein during preimplantation mouse development. Biology of reproduction. 2004; 70:1070-9

Cook, D, Gebski, V, Keech, A. Subgroup analysis in clinical trials. The Medical journal of Australia. 2004; 180:289-91

Cook, D, Dinudom, A, Young, J. Epithelial Na(+) channels and their regulation. Epithelial Transport of Ions in Health and Disease. 2004; Halle (Saale) 19-22 March 2003; 2003

2003

Weinhaus, A, Tabiin, M, Poronnik, P, Palma, C, Cook, D, Tuch, B. Insulin secretagogues, but not glucose, stimulate an increase in [Ca2+]i in the fetal human and porcine beta-cell. The Journal of clinical endocrinology and metabolism. 2003; 88:2753-2759

Fotia, A, Dinudom, A, Shearwin, K, Koch, J, Korbmacher, C, Cook, D, Kumar, S. The role of individual Nedd4-2 (KIAA0439) WW domains in binding and regulating epithelial sodium channels. Faseb Journal. 2003; 17:70-72

2002

Harding, E, Gibb, C, Johnson, M, Cook, D, Day, M. Developmental changes in the management of acid loads during preimplantation mouse development. Biology of reproduction. 2002; 67:1419-1429

Cummins, M, O'Mullane, L, Barden, J, Cook, D, Poronnik, P. Antisense co-suppression of G(alpha)(q) and G(alpha)(11) demonstrates that both isoforms mediate M(3)-receptor-activated Ca(2+) signalling in intact epithelial cells. Pflügers Archiv : European journal of physiology. 2002; 444:644-53

Cook, D, Kunzelman, K, Shreiber, R. Mechanisms for the inhibition of amilorie-sensitive Na+ absorption by extracellular nucleotides in mouse trachea. European Journal Of Applied Physiology. 2002; 444:220-226

Cook, D, Young, J. Towards a physiology of epithelial pathogens. European Journal Of Applied Physiology. 2002; 443:339-343

Poronnik, P, Cummins, M, O'Mullane, L, Cook, D. Use of adenoviruses to study isoform specificity of G-protein-receptor-coupled Ca2+ signaling in intact epithelial cells. Cell biochemistry and biophysics. 2002; 36:221-233

Konstas, A, Shearwin-Whyatt, L, Fotia, A, Degger, B, Riccardi, D, Cook, D, Korbacher, C, Kumar, S. Regulation of the epithelial sodium channel by N4WBP5A a novel Nedd4/Nedd4-2-interacting protein. Journal Of Biological Chemistry. 2002; 277,33:29406-29416

Fotia, A, Dinudom, A, Shearwin, K, Koch, J, Korbmacher, C, Cook, D, Kumar, S. The role of individual Nedd4-2 (KIAA0439) WW domains in binding and regulating epithelial sodium channels. Faseb Journal. 2002; 10.1096:1-22

2001

Dinudom, A, Harvey, K, Komwatana, P, Jolliffe, C, Young, J, Kumar, S, Cook, D. Roles of the C Termini of alpha, beta and subunits of epithelial Na+ channels (ENaC) in regulating ENaC and mediating its inhibition by cytosolic Na+. Journal of Biological Chemistry. 2001; 55:588-594

Harvey, K, Dinudom, A, Cook, D, Kumar, S. The Nedd4-like protein KIAA0439 is a potential regulator of the epithelial sodium channel. Journal of Biological Chemistry. 2001; Wyndham Anatole Dallas, Texas; 2001

Day, M, Winston, N, McConnell, J, Cook, D, Johnson, M. tiK+ toK+: an embryonic clock?. Reproduction, fertility, and development. 2001; 13:69-79

2000

Kunzelmann, K, Beesley, A, King, N, Karupiah, G, Young, J, Cook, D. Influenza virus inhibits amiloride-sensitive Na+ channels in respiratory epithelia. Proceedings of the National Academy of Sciences. 2000; 97(18):10282-10287

Bennett, M, Dutton, J, Poronnik, P, Li, G, Holding, C, Worthington, R, Vandenberg, R, Cook, D, Barden, J. P2x1 receptor membrane redistribution and down regulation visualised by using receptor-coupled green fluorescent protein chimeras. Neuropharmacology. 2000; 39:2054-2066

Cummins, M, OMullane, L, Barden, J, Cook, D, Poronnik, P. Purinergic responses in HT29 colonic epithelial cells are mediated by G protein (alpha)-subunits. Cell Calcium. 2000; 27(5):247-255

Cummins, M, OMullane, L, Barden, J, Cook, D, Poronnik, P. Purinergic responses in HT29 colonic epithelial cells are mediated by G protein @-subunits. Cell Calcium. 2000; 27:247-255

Cummins, M, Poronnik, P, OMullane, L, Cook, D. Studying heterotrimeric G-protein-linked signal transduction using replication-deficient adenoviruses. Immunology and Cell Biology. 2000; 78:375-386

Bennett, M, Li, G, Lee, E, Blair, D, Holding, C, Poronnik, P, Cook, D, Barden, J. The distribution of P2X receptor clusters on individual neurons in sympathetic ganglia and their redistribution on agonist activation. Journal of Biological Chemistry. 2000; 275:29107-29112

Carlin, S, Poronnik, P, Cook, D, Johnson, P, Black, J, Carpenter, L, Biden, T. An Antisense of Protein Kinase C- Inhibits Proliferation of Human Airway Smooth Muscle Cells. American Journal of Respiratory Cell and Molecular Biology. 2000; 23:555-559

Carlin, S, Poronnik, P, Cook, D, Carpenter, L, Biden, T, Johnson, P, Black, J. An antisense of protein kinase C-? inhibits proliferation of human airway smooth muscle cells. American Journal of Repiratory Cell and Molecular Biology. 2000; 23:555-559

Cook, D, Stojanov, T, O'Neill, C, Emerson, M, Harding, E, Travis, A, Bathgate, R, Lu, D. Characterization and functional significance of calcium transients in the 2-cell mouse embryo induced bu an autocrine growth factor. Journal of Biological Chemistry. 2000; 275:21905-21913

Dutton, J, Poronnik, P, Li, G, Holding, C, Worthington, R, Vandenberg, R, Cook, D, Barden, J, Bennett, M. P2X1 receptor membrane redistribution and down-regulation visualized by using receptor-coupled green fluorescent protein chimeras. Neuropharmacology. 2000; 39:2054-2066