Dr Paul Witting
D06 - Blackburn Building
The University of Sydney NSW 2006 Australia |
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Dr Paul Witting is a Senior Lecturer in the Discipline of Pathology at The University of Sydney and a career biomedical researcher. He has held ARC (2003-2007) and National Heart Foundation Fellowships (1999-2001) that financed post-doctoral terms at the ANZAC Research Institute and University of British Columbia (Canada), respectively. He has a proven track record in the field of bio-medical chemistry publishing 61 peer-reviewed papers (mean impact factor (IF) ~4.6 with >90% appearing in international journals); 4-book chapters; 9-invited review articles and one International Patent. Witting is first or senior author in ~65% of his papers and his work has been acknowledged with 1310 citations (mean citation/publication ~23 yielding an H-index ~23). He has been awarded ~A$3.7 Million in competitive funding from agencies including the ARC, NHF, Wellcome Trust, NHMRC Equipment Grants, The Ramaciotti, Bushell and CASS Foundations, Eli Lilly Diabetes Foundation, Diabetes Australia Research Trust and the University of Sydney Strategic Development Grants Scheme.
In July of 2008 Witting moved from the ANZAC Research Institute to take up a full time Senior Lectureship and establish the Redox Biology Group in the Discipline of Pathology, The University of Sydney. As part of this relocation Witting has been awarded over $175 K in start-up funding that includes a newly renovated laboratory with up-to-date equipment. The new facility combined with significant funding from the Heart Foundation and the ARC over the next 2-3 years provides an excellent environment for young researchers to be trained in biomedical techniques and explore the relationships between oxidative stress and disease
In July of 2008 Witting moved from the ANZAC Research Institute to take up a full time Senior Lectureship and establish the Redox Biology Group in the Discipline of Pathology, The University of Sydney. As part of this relocation Witting has been awarded over $175 K in start-up funding that includes a newly renovated laboratory with up-to-date equipment. The new facility combined with significant funding from the Heart Foundation and the ARC over the next 2-3 years provides an excellent environment for young researchers to be trained in biomedical techniques and explore the relationships between oxidative stress and disease
Dr Witting’s research interest is in exploring the relationship between oxidative stress and the evolution of tissue damage in the acute setting of stroke and myocardial infarct. He is also collaborating with clinicians in the Concord Hospital Burn Unit as he examines the relationship between severe burn and acute renal failure.
Current national competitive grants
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2009 |
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The fate of dietary selenium in vivo; a direct approach to linking chemical form with biological activity |
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| ARC Shared Research Support | $80,000 over 5 years |
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The Role of Snoring Vibrations in the Pathogenesis of Early Carotid Artery Atherosclerosis |
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| NHMRC Project Grants | $570,075 over 3 years |
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2008 |
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Cellular response to pro-oxidative myoglobin |
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| ARC Discovery Projects | $420,000 over 5 years |
2010
- Niknami, M, Vignarajan, S, Yao, M, Hua, S, Witting, P, Kita, Y, Shimizu, T, Sved, P, Patel, M, Dong, Q. Decrease in expression or activity of cytosolic phospholipase A(2)alpha increases cyclooxygenase-1 action: A cross-talk between key enzymes in arachidonic acid pathway in prostate cancer cells. Biochimica et biophysica acta. 2010; 1801:731-7
- Shanu, A, Parry, S, Wood, S, Rodas, E, Witting, P. The synthetic polyphenol tert-butyl-bisphenol inhibits myoglobin-induced dysfunction in cultured kidney epithelial cells. Free radical research. 2010; :
- Carter, E, Rayner, B, McLeod, A, Wu, L, Marshall, C, Levina, A, Aitken, J, Witting, P, Lai, B, Cai, Z, Vogt, S, Lee, Y, Chen, C, Tobin, M, Harris, H, Lay, P. Silicon nitride as a versatile growth substrate for microspectroscopic imaging and mapping of individual cells. Molecular bioSystems. 2010; :
- Wang, Y, Liu, H, McKenzie, G, Witting, P, Stasch, J, Hahn, M, Changsirivathanathamrong, D, Wu, B, Ball, H, Thomas, S, Kapoor, V, Celermajer, D, Mellor, A, Keaney, J, Hunt, N, Stocker, R. Kynurenine is an endothelium-derived relaxing factor produced during inflammation. Nature medicine. 2010; 16:279-85
- Liu, J, Obando, D, Schipanski, L, Groebler, L, Witting, P, Kalinowski, D, Richardson, D, Codd, R. Conjugates of Desferrioxamine B (DFOB) with Derivatives of Adamantane or with Orally Available Chelators as Potential Agents for Treating Iron Overload. Journal of medicinal chemistry. 2010; 53:1370-82
- Szuchman-Sapir, A, Pattison, D, Davies, M, Witting, P. Site-specific hypochlorous acid-induced oxidation of recombinant human myoglobin affects specific amino acid residues and the rate of cytochrome b(5)-mediated heme reduction. Free radical biology & medicine. 2010; 48:35-46
2009
- Sabaretnam, T, Harris, M, Kockx, M, Witting, P, Le Couteur, D, Kritharides, L. The Effects Of Hydrogen Peroxide And Apolipoprotein E Isoforms On Apolipoprotein E Trafficking In Hepg2 Cells. Clinical and experimental pharmacology & physiology. 2009; 36:e96-102
- Song, C, Hsu, K, Yamen, E, Yan, W, Fock, J, Witting, P, Geczy, C, Freedman, S. Serum amyloid A induction of cytokines in monocytes/macrophages and lymphocytes. Atherosclerosis. 2009; 207:374-83
- Reichelt, M, Shanau, A, Willems, L, Witting, P, Ellis, N, Blackburn, M, Headrick, J. Endogenous adenosine selectively modulates oxidant stress via the A1 receptor in ischemic hearts. Antioxidants & redox signaling. 2009; 11:2641-50
- Rayner, B, Hua, S, Sabaretnam, T, Witting, P. Nitric oxide stimulates myoglobin gene and protein expression in vascular smooth muscle. Biochemical Journal. 2009; 423:169-177
- Whiteman, I, Gervasio, O, Cullen, K, Guillemin, G, Jeong, E, Witting, P, Antao, S, Minamide, L, Bamburg, J, Goldsbury, C. Activated actin-depolymerizing factor/cofilin sequesters phosphorylated microtubule-associated protein during the assembly of alzheimer-like neuritic cytoskeletal striations. The Journal of neuroscience. 2009; 29:12994-13005
- Niknami, M, Dong, Q, Witting, P. Pitfalls in the use of arachidonic acid oxidation products to assign lipoxygenase activity in cancer cells. Free radical research. 2009; 43:951-6
- Niknami, M, Patel, M, Witting, P, Dong, Q. Molecules in focus: Cytosolic phospholipase A(2)-alpha. The international journal of biochemistry & cell biology. 2009; 41:994-7
- Dong, L, Freeman, R, Liu, J, Zobalova, R, Marin-Hernandez, A, Stantic, M, Rohlena, J, Valis, K, Rodriguez-Enriquez, S, Butcher, B, Goodwin, J, Brunk, U, Witting, P, Moreno-Sanchez, R, Scheffler, I, Ralph, S, Neuzil, J. Suppression of tumor growth in vivo by the mitocan alpha-tocopheryl succinate requires respiratory complex II. Clinical Cancer Research. 2009; 15:1593-1600
- Duong, T, Witting, P, Antao, S, Parry, S, Kennerson, M, Lai, B, Vogt, S, Lay, P, Harris, H. Multiple protective activities of neuroglobin in cultured neuronal cells exposed to hypoxia re-oxygenation injury. Journal of neurochemistry. 2009; 108:1143-1154
- Song, C, Shen, Y, Yamen, E, Hsu, K, Yan, W, Witting, P, Geczy, C, Freedman, S. Serum amyloid A may potentiate prothrombotic and proinflammatory events in acute coronary syndromes. Atherosclerosis. 2009; 202:596-604
- Hua, S, Song, C, Geczy, C, Freedman, S, Witting, P. A role for acute-phase serum amyloid A and high-density lipoprotein in oxidative stress, endothelial dysfunction and atherosclerosis. Redox Report. 2009; 14:187-196
2008
- Thomas, S, Witting, P, Drummond, G. Redox control of endothelial function and dysfunction: molecular mechanisms and therapeutic opportunities. Antioxidants & Redox Signaling. 2008; 10:1713-1765
- Szuchman-Sapir, A, Pattison, D, Ellis, N, Hawkins, C, Davies, M, Witting, P. Hypochlorous acid oxidizes methionine and tryptophan residues in myoglobin. Free radical biology & medicine. 2008; 45:789-98
- Dong, L, Low, P, Dyason, J, Wang, X, Prochazka, L, Witting, P, Freeman, R, Swettenham, E, Valis, K, Liu, J, Zobalova, R, Turanek, J, Spitz, D, Domann, F, Scheffler, I, Ralph, S, Neuzil, J. Alpha-tocopheryl succinate induces apoptosis by targeting ubiquinone-binding sites in mitochondrial respiratory complex II. Oncogene. 2008; 27:4324-4335
- Duong, T, Antao, S, Ellis, N, Myers, S, Witting, P. Supplementation with a synthetic polyphenol limits oxidative stress and enhances neuronal cell viability in response to hypoxia-re-oxygenation injury. Brain research. 2008; 1219:8-18
- Parry, S, Ellis, N, Li, Z, Maitz, P, Witting, P. Myoglobin induces oxidative stress and decreases endocytosis and monolayer permissiveness in cultured kidney epithelial cells without affecting viability. Kidney & blood pressure research. 2008; 31:16-28
- Nikinami, M, Patel, M, Witting, P, Dong, Q. Aberrant activation of arachidonic acid and eicosanoid pathways-targets for treating prostate cancer. Recent Patents on Endocrine, Metabolic & Immune Drug Discovery. 2008; 2:9-15
- Witting, P, Zeng, B, Wong, M, McMahon, A, Rayner, B, Sapir, A, Lowe, H, Freedman, S, Brieger, D. Polymorphonuclear leukocyte phagocytic function increases in plasminogen knockout mice. Thrombosis research. 2008; 122:674-82
2007
- Dong, L, Swettenham, E, Eliasson, J, Wang, X, Gold, M, Medunic, Y, Stantic, M, Low, P, Prochazka, L, Witting, P, Turanek, J, Akporiaye, E, Ralph, S, Neuzil, J. Vitamin E analogues inhibit angiogenesis by selective induction of apoptosis in proliferating endothelial cells: the role of oxidative stress. Cancer research. 2007; 67:11906-11913
- Neuzil, J, Witting, P, Kontush, A, Headrick, J. Role of vitamins E and C in nuclear factor-kB and nitric oxide signaling. In: Encyclopedia of Vitamin E. United States: CABI 2007. p. 0.
- Witting, P, Rayner, B, Wu, B, Ellis, N, Stocker, R. Hydrogen peroxide promotes endothelial dysfunction by stimulating multiple sources of superoxide anion radical production and decreasing nitric oxide bioavailability. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology. 2007; 20:255-268
- Neuzil, J, Widén, C, Gellert, N, Swettenham, E, Zobalova, R, Dong, L, Wang, X, Lidebjer, C, Dalen, H, Headrick, J, Witting, P. Mitochondria transmit apoptosis signalling in cardiomyocyte-like cells and isolated hearts exposed to experimental ischemia-reperfusion injury. Redox report : communications in free radical research. 2007; 12:148-162
2006
- Bhindi, R, Witting, P, McMahon, A, Khachigian, L, Lowe, H. Rat models of myocardial infarction. Pathogenetic insights and clinical relevance. Thrombosis and haemostasis. 2006; 96:602-10
- Witting, P, Harris, H, Rayner, B, Aitken, J, Dillon, C, Stocker, R, Lai, B, Cai, Z, Lay, P. The Endothelium-Derived Hyperpolarizing Factor, H(2)O(2), Promotes Metal-Ion Efflux in Aortic Endothelial Cells: Elemental Mapping by a Hard X-ray Microprobe. Biochemistry. 2006; 45:12500-12509
- Witting, P, Liao, W, Mathew, Z, Neuzil, J. Expression of human myoglobin in H9c2 cells enhances toxicity to added hydrogen peroxide. Biochemical and biophysical research communications. 2006; 348:485-93
- Hack, B, Witting, P, Rayner, B, Stocker, R, Headrick, J. Oxidant stress and damage in post-ischemic mouse hearts: effects of adenosine. Molecular and cellular biochemistry. 2006; 287:165-75
- de Haan, J, Witting, P, Stefanovic, N, Pete, J, Daskalakis, M, Kola, I, Stocker, R, Smolich, J. Lack of the antioxidant glutathione peroxidase-1 does not increase atherosclerosis in C57BL/J6 mice fed a high-fat diet. Journal of lipid research. 2006; 47:1157-67
- Rayner, B, Harris, H, Carter, E, Vogt, S, Cai, Z, Lai, B, Chin, C, Lee, Y, Lay, P, Witting, P. The use of synchrotron radiation to measure ion flux and cellular protein and lipid changes within cardiac ischemia reperfusion injury. Atherosclerosis Supplements. 2006; 7:587-587
- Wu, B, Kathir, K, Witting, P, Beck, K, Choy, K, Li, C, Croft, K, Mori, T, Tanous, D, Adams, M, Lau, A, Stocker, R. Antioxidants protect from atherosclerosis by a heme oxygenase-1 pathway that is independent of free radical scavenging. The Journal of experimental medicine. 2006; 203:1117-27
- Rayner, B, Duong, T, Myers, S, Witting, P. Protective effect of a synthetic anti-oxidant on neuronal cell apoptosis resulting from experimental hypoxia re-oxygenation injury. Journal of neurochemistry. 2006; 97:211-21
- McMahon, A, Duong, T, Brieger, D, Witting, P. Scope for antioxidants to enhance thrombolytic therapies in stroke patients. Future Cardiology. 2006; 2:659-665
- McMahon, A, Duong, T, Brieger, D, Witting, P. Is there potential for antioxidants to enhance thrombolysis therapy in patients with ischemic stroke?. Future Cardiology. 2006; 2:659-665
2005
- Swettenham, E, Witting, P, Salvatore, B, Neuzil, J. Alpha-tocopheryl succinate selectively induces apoptosis in neuroblastoma cells: potential therapy of malignancies of the nervous system?. Journal of neurochemistry. 2005; 94:1448-56
- Stapelberg, M, Gellert, N, Swettenham, E, Tomasetti, M, Witting, P, Procopio, A, Neuzil, J. Alpha-tocopheryl succinate inhibits malignant mesothelioma by disrupting the fibroblast growth factor autocrine loop: mechanism and the role of oxidative stress. Journal of biological chemistry. 2005; 280:25369-76
- Witting, P, Wu, B, Raftery, M, Southwell-Keely, P, Stocker, R. Probucol protects against hypochlorite-induced endothelial dysfunction: identification of a novel pathway of probucol oxidation to a biologically active intermediate. Journal of biological chemistry. 2005; 280:15612-8
- Rayner, B, Wu, B, Raftery, M, Stocker, R, Witting, P. Human S-nitroso oxymyoglobin is a store of vasoactive nitric oxide. Journal of biological chemistry. 2005; 280:9985-93
- Wang, X, Witting, P, Salvatore, B, Neuzil, J. Vitamin E analogs trigger apoptosis in HER2/erbB2-overexpressing breast cancer cells by signaling via the mitochondrial pathway. Biochemical and Biophysical Research Communications. 2005; 326:282-289
- Neuzil, J, Rayner, B, Lowe, H, Witting, P. Oxidative stress in myocardial ischaemia reperfusion injury: a renewed focus on a long-standing area of heart research. Redox report: communications in free radical research. 2005; 10:187-97
2004
- Deng, Y, Wu, B, Witting, P, Stocker, R. Probucol protects against smooth muscle cell proliferation by upregulating heme oxygenase-1. Circulation. 2004; 110:1855-60
- Rayner, B, Stocker, R, Lay, P, Witting, P. Regio- and stereo-chemical oxidation of linoleic acid by human myoglobin and hydrogen peroxide: Tyr(103) affects rate and product distribution. The Biochemical journal. 2004; 381:365-72
- Rayner, B, Wu, B, Raftery, M, Stocker, R, Witting, P. Regulation of vascular tone by S-nitroso-myoglobin. Redox report : communications in free radical research. 2004; 9:382-6
- Witting, P, Stocker, R. Ascorbic acid as an antioxidant in atherosclerosis. In: Vitamin C: Its Functions and Biochemistry in Animals and Plants. United States: Garland Publishing 2004. p. 261-290.
2003
- Choy, K, Deng, Y, Hou, J, Wu, B, Lau, A, Witting, P, Stocker, R. Coenzyme Q(10) supplementation inhibits aortic lipid oxidation but fails to attenuate intimal thickening in balloon-injured New Zealand white rabbits. Free radical biology & medicine. 2003; 35:300-9
- Weber, T, Dalen, H, Andera, L, Negre-Salvayre, A, Auge, N, Sticha, M, Lloret, A, Terman, A, Witting, P, Huguchi, M, Plasilova, M, Zivny, J, Gellert, N, Weber, C, Neuzil, J. Mitochondria play a central role in apoptosis induced by alpha-Tocopheryl succinate, an agent with antineoplastic activity: comparison with receptor-mediated pro-apoptotic signaling. Biochemistry. 2003; 42:4277-4291
2002
- Witting, P, Mauk, A, Lay, P. Role of tyrosine-103 in myoglobin peroxidase activity: kinetic and steady-state studies on the reaction of wild-type and variant recombinant human myoglobins with H(2)O(2). Biochemistry. 2002; 41:11495-503
- Andriambeloson, E, Witting, P. Chemical regulation of nitric oxide: a role for intracellular myoglobin?. Redox report : communications in free radical research. 2002; 7:131-6
2001
- Upston, J, Witting, P, Brown, A, Stocker, R, Keaney, J. Effect of vitamin E on aortic lipid oxidation and intimal proliferation after arterial injury in cholesterol-fed rabbits. Free radical biology & medicine. 2001; 31:1245-53
- Witting, P, Mauk, A, Douglas, D, Stocker, R. Reaction of human myoglobin and peroxynitrite: characterizing biomarkers for myoglobin-derived oxidative stress. Biochemical and biophysical research communications. 2001; 286:352-6
- Mashima, R, Witting, P, Stocker, R. Oxidants and antioxidants in atherosclerosis. Current opinion in lipidology. 2001; 12:411-8
2000
- Raitakari, O, McCredie, R, Witting, P, Griffiths, K, Letters, J, Sullivan, D, Stocker, R, Celermajer, D. Coenzyme Q improves LDL resistance to ex vivo oxidation but does not enhance endothelial function in hypercholesterolemic young adults. Free Radical Biology and Medicine. 2000; 28:1100-1105
- Lau, A, Witting, P, Chaufour, X, Celermajer, D, Pettersson, K, Stocker, R. Protective effects of probucol in two animal models of atherosclerosis. Redox Report. 2000; 5:116-118
