Associate Professor Robert Vandenberg
D06 - Blackburn Building
The University of Sydney NSW 2006 Australia |
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Associate Professor Vandenberg’s research interests have focused on understanding how neurotransmitter transporters work. These proteins play key roles in regulating neurotransmission in the brain and are also the targets of a number of theraputic drugs and drugs of abuse. The laboratory uses a number of state-of-the-art molecular and biophysical techniques to address questions as to how these proteins bind neurotransmitters, how drugs interact with and modulate transporters and how these processes go wrong in disease states such Alzheimer’s disease, Motor Neurone Disease and Ischemia following a stroke.
Current national competitive grants
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2009 |
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Drug binding Sites on Glycine Transporters |
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| NHMRC Project Grants | $478,500 over 3 years |
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2005 |
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Senior Research Fellowship |
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| NHMRC Research Fellowships | $696,250 over 6 years |
2010
- Connor, M, Vaughan, C, Vandenberg, R. N-Acyl amino acids and N-acyl neurotransmitter conjugates: neuromodulators and probes for new drug targets. British journal of pharmacology. 2010; 160:1857-71
- Ryan, R, Kortt, N, Sirivanta, T, Vandenberg, R. The position of an arginine residue influences substrate affinity and K coupling in the human glutamate transporter, EAAT1. Journal of neurochemistry. 2010; 114:565-75
- McKinzie, A, Ryan, R, Vandenberg, R. Site-directed mutagenesis in the study of membrane transporters. In: Methods in Molecular Biology: Membrane Transporters in Drug Discovery and Development Methods and Protocols. United States: Humana Press 2010. p. 277-293.
2009
- Edington, A, McKinzie, A, Reynolds, A, Kassiou, M, Ryan, R, Vandenberg, R. Extracellular loops 2 and 4 of GLYT2 are required for N-arachidonyl-glycine inhibition of glycine transport. The Journal of biological chemistry. 2009; 284:36424-30
- Habjan, S, Vandenberg, R. Modulation of Glutamate and Glycine Transporters by Niflumic, Flufenamic and Mefenamic Acids. Neurochemical research. 2009; 34:1738-47
- Liu, X, Mitrovic, A, Vandenberg, R. Glycine transporter 1 associates with cholesterol-rich membrane raft microdomains. Biochemical and Biophysical Research Communications. 2009; 384:530-534
- Huang, S, Ryan, R, Vandenberg, R. The role of cation binding in determining substrate selectivity of glutamate transporters. The Journal of biological chemistry. 2009; 284:4510-5
2008
- Vandenberg, R, Huang, S, Ryan, R. Slips, leaks and channels in glutamate transporters. Channels. 2008; 2:51-58
- Yang, Z, Aubrey, K, Alroy, I, Harvey, R, Vandenberg, R, Lynch, J. Subunit-specific modulation of glycine receptors by cannabinoids and N-arachidonyl-glycine. Biochemical Pharmacology. 2008; 76:1014-1023
2007
- Huang, S, Vandenberg, R. Mutations in Transmembrane Domains 5 and 7 of the Human Excitatory Amino Acid Transporter 1 Affect the Substrate-Activated Anion Channel. Biochemistry. 2007; 46:9685-92
- Vandenberg, R, Shaddick, K, Ju, P. Molecular basis for substrate discrimination by glycine transporters. The Journal of biological chemistry. 2007; 282:14447-14453
2006
- Wiles, A, Pearlman, R, Rosvall, M, Aubrey, K, Vandenberg, R. N-Arachidonyl-glycine inhibits the glycine transporter, GLYT2a. Journal of neurochemistry. 2006; 99:781-6
- Vandenberg, R. Mutational analysis of glutamate transporters. Handbook of experimental pharmacology. 2006; 175:113-35
2005
- Aubrey, K, Vandenberg, R, Clements, J. Dynamics of forward and reverse transport by the glial glycine transporter, glyt1b. Biophysical journal. 2005; 89:1657-68
- Vandenberg, R, Ryan, R. How and why are channels in transporters?. Science's STKE [electronic resource]: signal transduction knowledge environment. 2005; 2005:pe17
- Ryan, R, Vandenberg, R. A channel in a transporter. Clinical and experimental pharmacology & physiology. 2005; 32:1-6
2004
- Vandenberg, R, Ju, P, Aubrey, K, Ryan, R, Mitrovic, A. Allosteric modulation of neurotransmitter transporters at excitatory synapses. European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences. 2004; 23:1-11
- Liu, L, Markus, I, Vandenberg, R, Neilan, B, Murray, M, Burcher, E. Molecular identification and characterization of three isoforms of tachykinin NK(1)-like receptors in the cane toad Bufo marinus. American journal of physiology. Regulatory, integrative and comparative physiology. 2004; 287:R575-85
- Ju, P, Aubrey, K, Vandenberg, R. Zn2+ inhibits glycine transport by glycine transporter subtype 1b. The Journal of biological chemistry. 2004; 279:22983-91
- Ryan, R, Mitrovic, A, Vandenberg, R. The chloride permeation pathway of a glutamate transporter and its proximity to the glutamate translocation pathway. The Journal of biological chemistry. 2004; 279:20742-51
2003
- Pearlman, R, Aubrey, K, Vandenberg, R. Arachidonic acid and anandamide have opposite modulatory actions at the glycine transporter, GLYT1a. Journal of neurochemistry. 2003; 84:592-601
- Mitrovic, A, Vandenberg, R. Glutamate transporters: redefining the view of a transporter. Australian Biochemist. 2003; 34(3):8-11
- Ryan, R, Mitrovic, A, Vandenberg, R. Site-directed mutagenesis in the study of membrane transporters. Methods in molecular biology. 2003; 227:97-108
2002
- Moussa, C, Mitrovic, A, Vandenberg, R, Provis, T, Rae, C, Bubb, W, Balcar, V. Effects of L-glutamate transport inhibition by a conformationally restricted glutamate analogue (2S,1'S,2'R)-2-(carboxycyclopropyl)glycine (L-CCG III) on metabolism in brain tissue in vitro analysed by NMR spectroscopy. Neurochemical research. 2002; 27:27-35
- Ryan, R, Vandenberg, R. Distinct conformational states mediate the transport and anion channel properties of the glutamate transporter EAAT-1. Journal of Biological Chemistry. 2002; 277:13494-13500
2001
- Vandenberg, R, Aubrey, K. Glycine transport inhibitors as potential antipsychotic drugs. Expert Opinion On Emerging Therapeutic Targets. 2001; 10:182-189
- Aubrey, K, Vandenberg, R. N[3-(4-fluorophenyl)-3-(4'-phenylphenoxy) propyl]sarcosine (NFPS) is a selective persistent inhibitor of glycine transport. British Journal of Pharmacology. 2001; 86:110-119
- Mitrovic, A, Plesko, F, Vandenberg, R. Zn2+ inhibits the anion conductance of the glutamate transporter EAAT4. Journal of Biological Chemistry. 2001; 2:272-275
- Poulsen, M, Vandenberg, R. Niflumic acid modulates uncoupled substrate-gated conductances in the human glutamate transporter EAAT4. Journal of Physiology-London. 2001; 5:67-73
2000
- Dutton, J, Poronnik, P, Li, G, Holding, C, Worthington, R, Vandenberg, R, Cook, D, Barden, J, Bennett, M. P2X1 receptor membrane redistribution and down-regulation visualized by using receptor-coupled green fluorescent protein chimeras. Neuropharmacology. 2000; 39:2054-2066
- Aubrey, K, Mitrovic, A, Vandenberg, R. Molecular basis for proton regulation of glycine transport by glycine transporter subtype 1b. Molecular Pharmacology. 2000; 58:129-135
