Professor David G Allen
F13 - Anderson Stuart Building
The University of Sydney NSW 2006 Australia |
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The Laboratory of Muscle Cell Function analyses heart and skeletal muscle function both in health and disease. Our main approach has been to isolate single cells (ventricular muscle, skeletal muscle, pacemaker cells) and use fluorescent indicators to measure key intracellular ionic concentrations, for instance Ca2+, Na+ and pH together with indicators of function, such as force, shortening or electrical activity. Increasingly we use Western blotting, immunohistochemistry and expression systems to identify and localize proteins in the cells under study.
We also perform some whole animals studies, for instance running mice on treadmills and running wheels, to understand how exercise modifies muscle behaviour and how drugs modify disease processes.
We also perform some whole animals studies, for instance running mice on treadmills and running wheels, to understand how exercise modifies muscle behaviour and how drugs modify disease processes.
Current national competitive grants
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2010 |
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Molecular mechanisms of cardiac function and disease |
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| NHMRC Program Grant/Shared Research Support | $1,448,890 over 5 years |
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Intracellular calcium in intact muscle during fatigue and stretch-induced damage |
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| ARC Discovery Project | $285,000 over 3 years |
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2009 |
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The role of calcium and TRPC channels in sinoatrial node ageing |
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| NHMRC Project Grants | $370,125 over 3 years |
2010
- Allen, D, Westerblad, H. What limits exercise during high-intensity aerobic exercise?. European journal of applied physiology. 2010; :
- Allen, D, Gervasio, O, Yeung, E, Whitehead, N. Calcium and the damage pathways in muscular dystrophy. Canadian journal of physiology and pharmacology. 2010; 88:83-91
2009
- Reardon, T, Allen, D. Time to fatigue is increased in mouse muscle at 37A{degrees}C; the role of iron and ROS. The Journal of physiology. 2009; 587:4705-16
- Reardon, T, Allen, D. Iron injections in mice increase skeletal muscle iron content, induce oxidative stress and reduce exercise performance. Experimental Physiology. 2009; 94:720-730
- Allen, D. Fatigue in working muscles. Journal of applied physiology (Bethesda, Md. : 1985). 2009; 106:358-9
- Murphy, E, Allen, D. Why did the NHE inhibitor clinical trials fail?. Journal of Molecular and Cellular Cardiology. 2009; 46:137-141
- Westerblad, H, Allen, D. Cellular mechanisms of skeletal muscle fatigue. In: Human Muscle Fatigue. United Kingdom: Routledge 2009. p. 48-75.
2008
- Zhang, B, Yeung, S, Allen, D, Qin, L, Yeung, E. Role of the calcium-calpain pathway in cytoskeletal damage after eccentric contractions. Journal of applied physiology. 2008; 105:352-357
- Gervásio, O, Whitehead, N, Yeung, E, Phillips, W, Allen, D. TRPC1 binds to caveolin-3 and is regulated by Src kinase - role in Duchenne muscular dystrophy. Journal of Cell Science. 2008; 121:2246-2255
- Whitehead, N, Pham, C, Gervasio, O, Allen, D. N-Acetylcysteine ameliorates skeletal muscle pathophysiology in mdx mice. The Journal of Physiology. 2008; 586:2003-2014
- Allen, D, Lamb, G, Westerblad, H. Cellular mechanisms of skeletal muscle fatigue. In: Physiological Bases of Human Performance during Work and Exercise. United States: Churchill Livingstone 2008. p. 115-125.
- Ward, M, Williams, I, Chu, Y, Cooper, P, Ju, Y, Allen, D. Stretch-activated channels in the heart: contributions to length-dependence and to cardiomyopathy. Progress in biophysics and molecular biology. 2008; 97:232-249
- Allen, D, Lamb, G, Westerblad, H. Skeletal muscle fatigue: cellular mechanisms. Physiological Reviews. 2008; 88:287-332
- Allen, D, Lamb, G, Westerblad, H. Impaired calcium release during fatigue. Journal of applied physiology (Bethesda, Md. : 1985). 2008; 104:296-305
2007
- Ju, Y, Allen, D. Store-Operated Ca(2+) Entry and TRPC Expression; Possible Roles in Cardiac Pacemaker Tissue. Heart, lung & circulation. 2007; 16:349-55
- Williams, I, Xiao, X, Ju, Y, Allen, D. The rise of [Na(+)] (i) during ischemia and reperfusion in the rat heart-underlying mechanisms. Pfl��gers Archiv : European journal of physiology. 2007; 454:903-12
- Williams, I, Allen, D. THE ROLE OF REACTIVE OXYGEN SPECIES IN THE HEARTS OF DYSTROPHIN-DEFICIENT (MDX) MICE. American journal of physiology. Heart and circulatory physiology. 2007; 293:H1969-77
- Allen, D, Westerblad, H. Understanding muscle from its length. The Journal of physiology. 2007; 583:3-4
- Ju, Y, Chu, Y, Chaulet, H, Lai, D, Gervasio, O, Graham, R, Cannell, M, Allen, D. Store-operated Ca2+ influx and expression of TRPC genes in mouse sinoatrial node. Circulation research. 2007; 100:1605-1614
- Williams, I, Allen, D. Intracellular calcium handling in ventricular myocytes from mdx mice. American journal of physiology. Heart and circulatory physiology. 2007; 292:H846-55
- Korhonen, T, Bruton, J, Westerblad, H, Tavi, P, Aydin, J, Allen, D. Activation of Ca2+-dependent protein kinase II during repeated contractions in single muscle fibres from mouse is dependent on the frequency of sarcoplasmic reticulum Ca2+ release. ACTA PHYSIOLOGICA. 2007; 191:131-137
2006
- Whitehead, N, Streamer, M, Lusambili, L, Sachs, F, Allen, D. Streptomycin reduces stretch-induced membrane permeability in muscles from mdx mice. Neuromuscular disorders : NMD. 2006; 16:845-54
- Kizana, E, Ginn, S, Smyth, C, Boyd, A, Thomas, S, Allen, D, Ross, D, Alexander, I. Fibroblasts modulate cardiomyocyte excitability: implications for cardiac gene therapy. Gene therapy. 2006; 13:1611-5
- Moopanar, T, Xiao, X, Jiang, L, Chen, Z, Kemp, B, Allen, D. AICAR inhibits the Na+/H+ exchanger in rat hearts--possible contribution to cardioprotection. Pfl��gers Archiv : European journal of physiology. 2006; 453:147-56
- Tsoutsman, T, Chung, J, Doolan, A, Nguyen, L, Williams, I, Tu, E, Lam, L, Bailey, C, Rasko, J, Allen, D, Semsarian, C. Molecular insights from a novel cardiac troponin I mouse model of familial hypertrophic cardiomyopathy. Journal of molecular and cellular cardiology. 2006; 41:623-32
- Chu, Y, Allen, D, Ju, Y. The immunolocalization of TRPC1 in mouse sino-atrial node. Journal of Molecular and Cellular Cardiology. 2006; 41:735-735
- Whitehead, N, Yeung, E, Allen, D. Muscle damage in mdx (dystrophic) mice: role of calcium and reactive oxygen species. Clinical and experimental pharmacology & physiology. 2006; 33:657-62
- Allen, D. Why stretched muscles hurt--is there a role for half-sarcomere dynamics?. The Journal of physiology. 2006; 573:4
- Moopanar, T, Allen, D. The activity-induced reduction of myofibrillar Ca2+ sensitivity in mouse skeletal muscle is reversed by dithiothreitol. The Journal of physiology. 2006; 571:191-200
2005
- Allen, D, Whitehead, N, Yeung, E. Mechanisms of stretch-induced muscle damage in normal and dystrophic muscle: role of ionic changes. The Journal of physiology. 2005; 567:723-35
- Moopanar, T, Allen, D. Reactive oxygen species reduce myofibrillar Ca2+ sensitivity in fatiguing mouse skeletal muscle at 37 degrees C. The Journal of physiology. 2005; 564:189-99
- Ju, Y, Allen, D. Cyanide inhibits the Na+/Ca2+ exchanger in isolated cardiac pacemaker cells of the cane toad. Pflugers Archiv : European journal of physiology. 2005; 449:442-8
- Kizana, E, Ginn, S, Allen, D, Ross, D, Alexander, I. Fibroblasts can be genetically modified to produce excitable cells capable of electrical coupling. Circulation (Baltimore). 2005; 111:394-8
- Yeung, E, Whitehead, N, Suchyna, T, Gottlieb, P, Sachs, F, Allen, D. Effects of stretch-activated channel blockers on [Ca2+]i and muscle damage in the mdx mouse. Journal of Physiology-London. 2005; 562:367-380
2004
- Allen, D, Westerblad, H. Physiology. Lactic acid--the latest performance-enhancing drug. Science. 2004; 305:1112-3
- Yeung, E, Allen, D. Stretch-activated channels in stretch-induced muscle damage: role in muscular dystrophy. Clinical and experimental pharmacology and physiology. 2004; 31:551-6
- Allen, D. Skeletal muscle function: role of ionic changes in fatigue, damage and disease. Clinical and experimental pharmacology & physiology. 2004; 31:485-93
- Cooper, S, Maxwell, A, Kizana, E, Ghoddusi, M, Hardeman, E, Alexander, I, Allen, D, North, K. C2C12 co-culture on a fibroblast substratum enables sustained survival of contractile, highly differentiated myotubes with peripheral nuclei and adult fast myosin expression. Cell motility and the cytoskeleton. 2004; 58:200-11
- Westerblad, H, Allen, D. Cellular mechanisms of skeletal muscle fatigue. In: Molecular and Cellular Aspects of Muscle Contraction. Netherlands: Kluwer Academic / Plenum Publishers 2004. p. 563-570.
2003
- Xiao, X, Allen, D. The cardioprotective effects of Na+/H+ exchange inhibition and mitochondrial KATP channel activation are additive in the isolated rat heart. Pflügers Archiv : European journal of physiology. 2003; 447:272-9
- Ju, Y, Huang, W, Jiang, L, Barden, J, Allen, D. ATP modulates intracellular Ca2+ and firing rate through a P2Y1 purinoceptor in cane toad pacemaker cells. The Journal of physiology. 2003; 552:777-87
- Yeung, E, Head, S, Allen, D. Gadolinium reduces short-term stretch-induced muscle damage in isolated mdx mouse muscle fibres. The Journal of physiology. 2003; 552:449-58
- Ju, Y, Wu, M, Chaulet, H, Marciniec, T, Graham, R, Allen, D. IGF-1 enhances a store-operated Ca2+ channel in skeletal muscle myoblasts: involvement of a CD20-like protein. Journal of cellular physiology. 2003; 197:53-60
- Ju, Y, Allen, D. Early effects of metabolic inhibition on intracellular Ca2+ in toad pacemaker cells: involvement of Ca2+ stores. American journal of physiology. Heart and circulatory physiology. 2003; 284:H1087-94
- Allen, D, Xiao, X. Role of the cardiac Na+/H+ exchanger during ischemia and reperfusion. Cardiovascular research. 2003; 57:934-41
- Xiao, X, Allen, D. The role of endogenous angiotensin II in ischaemia, reperfusion and preconditioning of the isolated rat heart. Pflügers Archiv : European journal of physiology. 2003; 445:643-50
- Tavi, P, Allen, D, Niemela, P, Vuolteenaho, O, Weckstrom, M, Westerblad, H. Calmodulin kinase modulates Ca2+ release in mouse skeletal muscle. Journal Of Physiology-London. 2003; 551.1:5-12
- Yeung, E, Ballard, H, Bourreau, J, Allen, D. Intracellular sodium in mammalian muscle fibers after eccentric contractions. Journal Of Applied Physiology. 2003; 94:2475-2482
2002
- Yeung, E, Balnave, C, Ballard, H, Bourreau, J, Allen, D. Development of T-tubular vacuoles in eccentrically damaged mouse muscle fibres. Journal Of Physiology-London. 2002; 540.2:581-592
- Allen, D, Lannergren, J, Westerblad, H. Intracellular ATP measured with lucferin/luciferase in isolated single skeletal muscle fibres. Pflugers Archiv-European Journal Of Physiology. 2002; 443:836-842
- Allen, D, Westerblad, H, Lannergren, J. Muscle fatigue: Lactic acid or inorganic phosphate the Major cause?. News In Physiological Sciences. 2002; 17:17-21
- Allen, D, Westerblad, H, Kabbarra, A. Muscle Fatigue: The role of intracellular calcium stores. Journal Of Applied Physiology. 2002; 27:83-96
- Westerblad, H, Allen, D. Recent advances in the understanding of skeletal muscle fatigue. Current Opinion In Rheumatology. 2002; 14:648-652
2001
- Allen, D, Westerblad, H. Role of phosphate and calcium stores in muscle fatigue. The Journal of physiology. 2001; 536:657-65
- Ju, Y, Allen, D. Does Ca2+ release from the sarcoplasmic reticulum influence heart rate?. Clinical and Experimental Pharmacology and Physiology. 2001; 14:270S-275S
- Allen, D. Eccentric Muscle damage: mechanisms of early reduction of force. Acta Physiologica Scandinavica. 2001; 40:13002-13014
- Allen, D, Xiao, X. Na+ entry during ischemia, reperfusion and preconditioning. Cardiovascular Research. 2001; 86:678-682
- Kabbara, A, Allen, D. The use of the inidicator fluo-FN to measure sarcoplasmic reticulum calcium in single muslce fibres of the cane toad. Journal of Physiology-London. 2001; Sydney Australia; 2001
2000
- Ju, Y, Allen, D. The mechanisms of sarcoplasmic reticulum Ca2+ release in toad pacemaker cells. Journal of Physiology. 2000; 525.3:695-705
- Allen, D, Westerblad, H, Bruton, J, Lannergren, J. Functional significance of Ca2+ in long lasting fatigue of skeletal muscle. European Journal of Applied Physiology. 2000; 83:166-174
- Allen, D, Xiao, X. Activity of the Na+/H+ exchanger is critical to reperfusion damage and preconditioning in the isolated rat heart. Cardiovascular Research. 2000; 48:244-253
- Allen, D, MacIntosh, B. Contractile Changes and Mechanisms of Muscle Fatigue. In: Biomechanics and Biology of Movement. Champaign IL USA: Human Kinetics 2000. p. 365-384.
- Ju, Y, Allen, D. The distribution of calcium in toad cardiac pacemaker cells during spontaneous firing. European Journal of Physiology. 2000; 441:219-227
- Allen, D, Nguyen, L, Stephenson, G, Kabbara, A. Intracellular calcium during fatigue of cane toad skeletal muscle in the absence of glucose. Journal of Muscle Research and Cell Motility. 2000; 21:481-489
- Allen, D, Hawke, T, Lindinger, M. Paraxanthine, a caffeine metabolite, dose dependently increases [Ca2+]i in skeletal muscle. Journal of Applied Physiology. 2000; 89:2312-2317
- Allen, D, Xiao, X. Activity of the Na+/h+ exchanger contributes to cardiac damage following ischaemia and reperfusion. Clinical and Experimental Pharmacology and Physiology. 2000; 27:727-733
