Professor Cristobal Dos Remedios

T:  +61 2 9351 3209 F:  +61 2 9351 6546  Personal Homepage F13 - Anderson Stuart Building The University of Sydney NSW 2006 Australia

• Research interests
• Competitive grants
• Publications

Research interests

Current national competitive grants

Publications

2010

Kooij, V, Saes, M, Jaquet, K, Zaremba, R, Foster, D, Murphy, A, Dos Remedios, C, van der Velden, J, Stienen, G. Effect of troponin I Ser23/24 phosphorylation on Ca2+-sensitivity in human myocardium depends on the phosphorylation background. Journal of Molecular and Cellular Cardiology. 2010; 48:954-963

Lu, Z, Xu, X, Hu, X, Lee, S, Traverse, J, Zhu, G, Fassett, J, Tao, Y, Zhang, P, dos Remedios, C, Pritzker, M, Hall, J, Garry, D, Chen, Y. Oxidative stress regulates left ventricular PDE5 expression in the failing heart. Circulation. 2010; 121:1474-83

Kong, S, Hu, Y, Ho, J, Ikeda, S, Polster, S, John, R, Hall, J, Bisping, E, Pieske, B, dos Remedios, C, Pu, W. Heart failure-associated changes in RNA splicing of sarcomere genes. Circulation: Cardiovascular Genetics. 2010; 3:138-146

Kooij, V, Boontje, N, Zaremba, R, Jaquet, K, Dos Remedios, C, Stienen, G, van der Velden, J. Protein kinase C alpha and epsilon phosphorylation of troponin and myosin binding protein C reduce Ca(2+) sensitivity in human myocardium. Basic research in cardiology. 2010; 105:289-300

2009

van Dijk, S, Holewijn, R, Tebeest, A, Dos Remedios, C, Stienen, G, van der Velden, J. A piece of the human heart: variance of protein phosphorylation in left ventricular samples from end-stage primary cardiomyopathy patients. Journal of muscle research and cell motility. 2009; 30:299-302

Lal, S, Brown, A, Nguyen, L, Braet, F, Dyer, W, Dos Remedios, C. Using antibody arrays to detect microparticles from acute coronary syndrome patients based on CD antigen expression. Molecular & cellular proteomics : MCP. 2009; 8:799-804

van Dijk, S, Dooijes, D, dos Remedios, C, Michels, M, Lamers, J, Winegrad, S, Schlossarek, S, Carrier, L, ten Cate, F, Stienen, G, van der Velden, J. Cardiac myosin-binding protein C mutations and hypertrophic cardiomyopathy: haploinsufficiency, deranged phosphorylation, and cardiomyocyte dysfunction. Circulation. 2009; 119:1473-1483

Krüger, M, Kötter, S, Grützner, A, Lang, P, Andresen, C, Redfield, M, Butt, E, Dos Remedios, C, Linke, W. Protein Kinase G Modulates Human Myocardial Passive Stiffness by Phosphorylation of the Titin Springs. Circulation research. 2009; 104:87-94

Messer, A, Gallon, C, McKenna, W, Dos Remedios, C, Marston, S. The use of phosphate-affinity SDS-PAGE to measure the cardiac troponin I phosphorylation site distribution in human heart muscle. Proteomics Clinical Applications. 2009; 3:1371-1382

Ho, J, Stefani, M, dos Remedios, C, Charleston, M. A model selection approach to discover age-dependent gene expression patterns using quantile regression models. BMC Genomics. 2009; 10:S16

Estigoy, C, Ponten, F, Odeberg, J, Herbert, B, Guilhaus, M, Charleston, M, Ho, J, Cameron, D, dos Remedios, C. Intercalated discs: multiple proteins perform multiple functions in non-failing and failing human hearts. Biophysical Reviews. 2009; 1:43-49

2008

Fermin, D, Barac, A, Lee, S, Polster, S, Hannenhalli, S, Bergemann, T, Grindle, S, Dyke, D, Pagani, F, Miller, L, Tan, S, Dos Remedios, C, Cappola, T, Margulies, K, Hall, J. Sex and age dimorphism of myocardial gene expression in nonischemic human heart failure. Circulation: Cardiovascular genetics. 2008; 1:117-125

Martinez-Neira, R, Dos Remedios, C, Mackay, I. An actin-myosin functional assay for analysis of smooth muscle (anti-microfilament) autoantibodies in human plasma. Journal of immunological methods. 2008; 338:63-6

Ho, J, Stefani, M, Dos Remedios, C, Charleston, M. Differential variability analysis of gene expression and its application to human diseases. Bioinformatics. 2008; 24:i390-i398

Gan, B, Nosworthy, N, McKenzie, D, Dos Remedios, C, Bilek, M. Plasma immersion ion implantation treatment of polyethylene for enhanced binding of active horseradish peroxidase. Journal of biomedical materials research. Part A. 2008; 85:605-10

Hamdani, N, Kooij, V, van Dijk, S, Merkus, D, Paulus, W, Remedios, C, Duncker, D, Stienen, G, van der Velden, J. Sarcomeric dysfunction in heart failure. Cardiovascular Research. 2008; 77:649-658

Lui, P, Brown, A, Wu, B, Velauthen, C, Lin, M, Thompson, J, Braet, F, Dyer, W, Macdonald, P, Hayward, C, Adelstein, S, dos Remedios, C. Use of antibody microarrays in the analysis of inflammation, autoimmunity, viral infection, and cancer metastases. In: Clinical Proteomics: From Diagnosis to Therapy. United States: John Wiley & Sons 2008. p. 571-592.

dos Remedios, C, Chhabra, D. Foreword. In: Actin-binding proteins and disease. United Kingdom: Springer 2008. p. v-vii.

dos Remedios, C, Nosworthy, N. The role of PIP2 in actin, actin-binding proteins and disease. In: Actin-binding proteins and disease. United Kingdom: Springer 2008. p. 290-297.

Hamdani, N, de Waard, M, Messer, A, Boontje, N, Kooij, V, van Dijk, S, Versteilen, A, Lamberts, R, Merkus, D, Dos Remedios, C, Duncker, D, Borbely, A, Papp, Z, Paulus, W, Stienen, G, Marston, S, van der Velden, J. Myofilament dysfunction in cardiac disease from mice to men. Journal of muscle research and cell motility. 2008; 29:189-201

Brown, A, Lattimore, J, McGrady, M, Sullivan, D, Dyer, W, Braet, F, dos Remedios, C. Stable and unstable angina: Identifying novel markers on circulating leukocytes. PROTEOMICS - Clinical Applications. 2008; 2:90-98

Stefani, M, Tsubakihara, M, Allen, P, Macdonald, P, dos Remedios, C. Actin and its binding proteins in heart failure. In: Actin-binding proteins and disease. United Kingdom: Springer 2008. p. 318-334.

Ho, J, Koundinya, R, Caetano, T, dos Remedios, C, Charleston, M. Inferring differential leukocyte activity from antibody microarrays using a latent variable model. Genome informatics. International Conference on Genome Informatics. 2008; 21:126-137

dos Remedios, C, Chhabra, D. Actin-Binding Proteins and Disease. United Kingdom: Springer, 2008.

Chhabra, D, dos Remedios, C. Actin: an overview of its structure and function. In: Actin-binding proteins and disease. United Kingdom: Springer 2008. p. 1-16.

2007

Dos Remedios, C. New and Notable: The regulation of muscle contraction: As in life, it keeps getting more complex. Biophysical journal. 2007; 93:4097-8

Nicolau, D, Solana, G, Kekic, M, Fulga, F, Mahanivong, C, Wright, J, dos Remedios, C. Surface hydrophobicity modulates the operation of actomyosin-based dynamic nanodevices. Langmuir : the ACS journal of surfaces and colloids. 2007; 23:10846-10854

Zaremba, R, Merkus, D, Hamdani, N, Lamers, J, Paulus, W, dos Remedios, C, Duncker, D, Stienen, G, van der Velden, J. Quantitative analysis of myofilament protein phosphorylation in small cardiac biopsies. Proteomics - Clinical Applications. 2007; 1:1285 - 1290

Ho, J, Nosworthy, N, Bilek, M, Gan, B, McKenzie, D, Chu, P, dos Remedios, C. Plasma-Treated Polyethylene Surfaces for Improved Binding of Active Protein. Plasma Processes and Polymers. 2007; 4:583-590

2006

Gorbatyuk, V, Nosworthy, N, Robson, S, Bains, N, Maciejewski, M, dos Remedios, C, King, G. Mapping the phosphoinositide-binding site on chick cofilin explains how PIP2 regulates the cofilin-actin interaction. Molecular Cell. 2006; 24:511-522

Narolska, N, Piroddi, N, Belus, A, Boontje, N, Scellini, B, Deppermann, S, Zaremba, R, Musters, R, Dos Remedios, C, Jaquet, K, Foster, D, Murphy, A, van Eyk, J, Tesi, C, Poggesi, C, van der Velden, J, Stienen, G. Impaired Diastolic Function After Exchange of Endogenous Troponin I with C-Terminal Truncated Troponin I in Human Cardiac Muscle. Circulation research. 2006; 99:1012-20

Steel, B, McKenzie, D, Bilek, M, Nosworthy, N, Dos Remedios, C. Nanosecond responses of proteins to ultra-high temperature pulses. Biophysical journal. 2006; 91:L66-8

Dedova, I, Nikolaeva, O, Safer, D, De La Cruz, E, dos Remedios, C. Thymosin beta4 induces a conformational change in actin monomers. Biophysical journal. 2006; 90:985-92

Zhang, S, Yao, C, dos Remedios, C. The Amazing Human Body: the anatomical display of real human bodies. Australia: Redtail, 2006.

2005

Chhabra, D, dos Remedios, C. Cofilin, actin and their complex observed in vivo using fluorescence resonance energy transfer. Biophysical journal. 2005; 89:1902-8

Chhabra, D, Nosworthy, N, dos Remedios, C. The N-terminal fragment of gelsolin inhibits the interaction of DNase I with isolated actin, but not with the cofilin-actin complex. Proteomics. 2005; 5:3131-6

Lui, R, Tsubakihara, M, Macdonald, P, Hayward, C, dos Remedios, C. Analysing inflammation in heart failure using microarray platforms. Journal of Molecular and Cellular Cardiology. 2005; 38:854-855

Jiang, L, Bardini, M, Keogh, A, dos Remedios, C, Burnstock, G. P2X1 receptors are closely associated with connexin 43 in human ventricular myocardium. International journal of cardiology. 2005; 98:291-7

Martinez-Neira, R, Kekic, M, Nicolau, D, dos Remedios, C. A novel biosensor for mercuric ions based on motor proteins. Biosensors & Bioelectronics. 2005; 20:1428-1432

Martinez-Neira, R, Kekic, M, Nicolau, D, dos Remedios, C. A novel biosensor for mercuric ions based on motor proteins. Biosensors & bioelectronics. 2005; 20:1428-1432

Chhabra, D, dos Remedios, C. Intracellular fluorescence resonance energy transfer. Biophysical Journal. 2005; 89:1902-1908

Biek, M, McKenzie, D, Nosworthy, N, DAVIES, K, MORROW, R, THORDARSON, P, GAN, B, dos Remedios, C. Functional attachment of horse radish peroxidase to plasma-treated surfaces. Smart Materials III. 2005; Univ New South Wales Dec 13; 2004

Ramdutt, D, Lui, R, Boswell, R, dos Remedios, C, Charles, C, Bilek, M, McKenzie, D. Development of the nanotiter plate for use in antibody and cell array technologies. SPIE. 2005; UNSW; 2004

Kekic, M, Martinez, R, dos Remedios, C, Nicolau, D. An acto/myosin in vitro motility assay as a motion based sensor. Biophysical Journal. 2005; 88:503A-503A

2004

Dedova, I, Nikolaeva, O, Mikhailova, V, dos Remedios, C, Levitsky, D. Two opposite effects of cofilin on the thermal unfolding of F-actin: a differential scanning calorimetric study. Biophysical chemistry. 2004; 110:119-28

Lal, S, Lui, R, Nguyen, L, Macdonald, P, Denyer, G, dos Remedios, C. Increases in leukocyte cluster of differentiation antigen expression during cardiopulmonary bypass in patients undergoing heart transplantation. Proteomics. 2004; 4:1918-26

Borovikov, Y, Dedova, I, dos Remedios, C, Vikhoreva, N, Vikhorev, P, Avrova, S, Hazlett, T, Van Der Meer, B. Fluorescence depolarization of actin filaments in reconstructed myofibers: the effect of S1 or pPDM-S1 on movements of distinct areas of actin. Biophysical journal. 2004; 86:3020-9

Steel, B, Bilek, M, dos Remedios, C, McKenzie, D. Apparatus for exposing cell membranes to rapid temperature transients. European biophysics journal : EBJ. 2004; 33:117-20

Dedova, I, Avrova, S, Vikhoreva, N, Vikhorev, R, Hazlett, T, Van der Meer, W, Dos Remedios, C, Borovikov, I. [Conformational changes of actin induced by strong or weak myosin subfragment-1 binding]. Tsitologiia. 2004; 46:719-734

dos Remedios, C. AES is reborn as AEPS. Proteomics. 2004; 4:1857

dos Remedios, C. The 32nd European Muscle Conference. Journal of muscle research and cell motility. 2004; 25:3-5

Tsubakihara, M, Williams, N, Keogh, A, dos Remedios, C. Comparison of gene expression between left atria and left ventricles from non-diseased humans. Proteomics. 2004; 4:261-70

2003

Dunn, T, Heller, C, McCarthy, S, Dos Remedios, C. Anatomical study of the "trochanteric bursa". Clinical anatomy. 2003; 16:233-240

dos Remedios, C, Chhabra, D, Kekic, M, Dedova, I, Tsubakihara, M, Berry, D, Nosworthy, N. Actin binding proteins: regulation of cytoskeletal microfilaments. Physiological reviews. 2003; 83:433-73

Jiang, L, Huang, Y, Hunyor, S, dos Remedios, C. Cardiomyocyte apoptosis is associated with increased wall stress in chronic failing left ventricle. European Heart Journal. 2003; 24:742-751

Steel, B, Bilek, M, dos Remedios, C, McKenzie, D. Apparatus for exposing cell membranes to rapid temperature transients. European Biophysics Journal With Biophysics Letters. 2003; 33:117-120

Ng, D, Court, N, dos Remedios, C, Bogoyevitch, M. Activation of signal transducer and activator of transcription (STAT) pathways in failing human hearts. Cardiovascular Research. 2003; 57:333-346

2002

Lal, S, Christopherson, R, dos Remedios, C. Antibody arrays: an embryonic but rapidly growing technology. Drug discovery today. 2002; 7:S143-S149

Thomas, D, dos Remedios, C. Molecular Interactions of Actin; Actin-Myosin Interaction & Actin-Based Regulation. Germany: Springer-Verlag, 2002.

Steel, B, Bilda, Z, McKenzie, D, dos Remedios, C. A technique for microsecond heating & cooling of a thin (submicron) biological sample. European Biophysics Journal With Biophysics Letters. 2002; 31:378-382

Court, N, dos Remedios, C, Cordell, J, Bogoyevitch, M. Cardiac expression & subcellular localization of the p38 Mitogen-activated protein kinase member, stress-activated protein kinases-3 (SAPK3). Journal Of Molecular And Cellular Cardiology. 2002; 34:413-426

Heller, C, Marucci, D, Dunn, T, Barr, E, Houang, M, dos Remedios, C. Inguinal canal 'Lipoma'. Clinical Anatomy. 2002; 15:280-285

Bains, N, Gorbatyuk, V, Nosworthy, N, Robson, S, Maciejewski, M, dos Remedios, C, King, G. Letter to the Editor: Backbone & side-chain 1H, 15N, and 13C assignments for chick cofilin. Journal Of Biomolecular Nmr. 2002; 22:193-194

Mahanivong, C, Wright, J, Kekic, M, Pham, D, dos Remedios, C, Nicolau, D. Manipulation of the motility of protein molecular motors on microfabricated substrates. Biomedical Microdevices. 2002; 4:02:111-116

Chhabra, D, Bao, S, dos Remedios, C. The distribution of cofilin and DNase I in vivo. Cell Research. 2002; 12(3-4):207-214

2001

Belov, L, de la Vega, O, dos Remedios, C, Mulligan, S, Christopherson, R. Immunophenotyping of leukemias using a cluster of differentiation antibody microarray. Cancer research. 2001; 61:4483-9

dos Remedios, C, Thomas, D. An overview of actin structure and actin-binding proteins. Molecular Interactions of Actin; Actin structure and actin-binding proteins. 2001; 19:415-425

Heinke, M, Yao, M, Chang, D, Einstein, R, dos Remedios, C. Apoptosis of Ventricular and Atrial Myocytes from Pacing-Induced Canine Heart Failure. Cardiovascular Research. 2001; 6:389-392

Jiang, L, Tsubakihara, M, Heinke, M, Phillips, W, dos Remedios, C, Nosworthy, N, Yao, M. Heart failure and apoptosis: Electrophoretic methods support data from micro- and macro-arrays. A critical review of genomics and proteomics. Proteomics. 2001; 52:863-870

Belov, L, de la Vega, O, dos Remedios, C, Mulligan, S, Christopherson, R. Immunophenotyping of leukemias using a cluster of differentiation antibody microarray1. In: Cancer Research. Usa: Wb Saunders Company 2001. p. 558-570.

Berry, D, Keogh, A, dos Remedios, C. Nuclear membrane proteins in failing human dilated cardiomyopathy. Proteomics. 2001; 142:2147-2150

Kekic, M, Nosworthy, N, Dedova, I, Collyer, C, dos Remedios, C. Regulation of the cytoskeleton assembly: a role for a ternary complex of actin with two actin-binding proteins. Results and Problems in Cell Differentiation. 2001; 32:165-179

Moens, P, dos Remedios, C. Analysis of models of F-Actin using fluorescence resonance energy transfer spectroscopy. Molecular Interactions of Actin: Actin structure and actin-binding proteins. 2001; 194:189-203

Heinke, M, Yao, M, Chang, D, Einstein, R, dos Remedios, C. Apoptosis of ventricular and atrial myocytes from pacinmg-induced canine heart failure. Cardiovascular Research. 2001; 6:200-203

2000

Berry, D, Balcar, V, Barden, J, Keogh, A, dos Remedios, C. Determination of P2X1 (alpha)-sarcoglycan (adhalin) expression levels in failing human dilated cardiomyopathic left ventricles. Electrophoresis. 2000; 21 (170:3857-3862

Hambly, B, Walsh, J, dos Remedios, C. Electrophoresis - a multidisciplinary and unifying technology. Electrophoresis. 2000; 21:3781-3783

Chhabra, D, Nosworthy, N, dos Remedios, C. The role of ATP, ADP and divalent cations in the formation of binary and ternary complexes of actin, cofilin and DNase I. Electrophoresis. 2000; 21 (17):3863-3869